PathFinder
Pathways, partners
and gene counting: Genetic techniques are commonly used to learn about
the relationship between the products of genes and their functions. In
this section, working with Brewer's yeast, you'll discover how to take
a disparate set of mutants and determine how many genes are represented
in the mutant collection, in what order they act, and how to discover
new members of the pathway. You'll also be exposed to the vagaries of
meiosis and its shuffling effect on genes combinations.
Handouts:
Yeast facts (.pdf)
Yeast mating type (.pdf)
Notebook example: (pdf)
Tutorials (These may be a little outdated; first check the internal tutorials in the Show Me menu):
Starting off and finding strain phenotypes
Heat shift experiments to determine cell cycle arrest
To get the Shockwave
plug-in that is required to run the simulations, click the icon below.
You'll need to follow a series of installation instructions; just do as
your told and click 'yes' whenever it asks your permission to do anything.
It's a big (1Meg) download, but you'll only need to do it once. You may
already have it installed; if you try the simulation, it will either run
OK or take you through the download process. Netscape browser is preferred;
if you run into difficulties with Internet Explorer, you might want to
try uninstalling the Shockwave plug-in first.
