MCB422

Bad notebook: A how-not-to guide for failing MCB422

In order to further delineate our expectations in terms of presenting your work, I asked a student, who has seen a lot of notebooks go by, to produce something shameful that incorporates some embarrassments to avoid. I edited her initial work, and our joint effort follows:

Student's comments: Besides for the control explanation, I tried not to include bad logic, just bad presentation... it definitely includes some of the most common problems from last year, and things like being specific and doing controls are good to know!!

Rationale: To accomplish this goal, we will need to plate phage and harvest the plaques that are growing.  These will be the ones that are new mutations of the opposite sign.
Commentary: More of a protocol than principles... it gives the bottom line of the rationale but misses all of the logic behind it. 
For laughs and giggles, here's Webster's II New College Dictionary on rationale:
1. The fundamental reasons for something: BASIS.
2. An exposition of the guiding principles or justifications.
Note that I have decided to summarily execute, with extreme prejudice, those who refuse to 'get' rationale this year.

Observations: Poured one plate of the starting mutant.  I observed some plaques growing and harvested them into the fridge.  To make sure that these were the new mutations of the opposite sign (like the ones discussed in the rationale) and not contaminants, I plated several control plates with higher dilution factors.  Since no plaques grew on these plates, I realized that there were no contaminants in the experiment and the ones of the main plate must have been new mutations of the opposite sign.
Commentary: Observations do not include critical information: dilution factor, B or K, an actual number of plaques, what kind of plaques were harvested, etc.
"Controls" were random tests at random dilutions.
People, the point of a scientific write-up is that if you died suddenly, an alien could walk in to the lab, pick up your notebook and resume your experiments. While we will not be enforcing THAT level of completeness, we should be able to deduce the key features of the experiment at hand without re-animating your brain. Do NOT assume you are talking to someone who knows more than you do; EXPLAIN yourself. This will make you a Great Communicator no matter where your life path leads you; few people complain about something being 'too clear' or having understood 'too well', myself and the T.A.s included!

Conclusions: The phage in my fridge numbered 3-7 contain new mutations of the opposite sign of the starting mutation.
Commentary: Conclusion is a one-sentence statement of the outcome and does not explain how it was arrived at (first person to comment on ending sentence in 'at' earns first -25 point notebook deduction!).

Final comment: Spellcheckers have been invented. Use it! Please do us the simple courtesy of spelling correctly; even if you can't, your computer can.