MCB422 Bad notebook: A how-not-to guide for failing MCB422 In order to further delineate our expectations in terms of presenting your work, I asked a student, who has seen a lot of notebooks go by, to produce something shameful that incorporates some embarrassments to avoid. I edited her initial work, and our joint effort follows: Student's comments: Besides for the control explanation, I tried not to include bad logic, just bad presentation... it definitely includes some of the most common problems from last year, and things like being specific and doing controls are good to know!! Rationale: To accomplish this goal, we will need to plate
phage and harvest the plaques that are growing. These will
be the ones that are new mutations of the opposite sign. Observations: Poured one plate of the starting
mutant. I
observed some plaques growing and harvested them into the fridge. To
make sure that these were the new mutations of the opposite sign
(like the ones discussed in the rationale) and not contaminants,
I plated several control plates with higher dilution factors. Since
no plaques grew on these plates, I realized that there were no
contaminants in the experiment and the ones of the main plate must
have been new mutations of the opposite sign. Conclusions: The phage in my fridge numbered 3-7 contain
new mutations of the opposite sign of the starting mutation. Final comment: Spellcheckers have been invented. Use it! Please do us the simple courtesy of spelling correctly; even if you can't, your computer can.
|