| Date |
Class and Project Focus |
Assignment / reading due |
| Aug. 23 |
Project 1: Introduction to course |
None |
| Aug. 25 |
Project 2: Isolation of Plasmid DNA: Exercises A,B,C: Solutions (Appendices) |
Questions for Project 1 due, Prelab for Project 2 due, READ Primrose pp 8-11, 43-53. |
| Aug. 30 |
Exercise D: the Qiagen midiprep |
|
| Sept. 1 |
Exercises E, F, G: Quantifying DNA |
|
| Sept. 6 |
Labor Day Holiday |
|
| Sept. 8 |
Exercises H, I : Restriction digests |
|
| Sept. 13 |
Project 3: Subcloning Plasmid DNA:Exercises A, B: Shotgun cloning |
Prelab Project 3 due, READ Primrose pp 26-42 |
| Sept. 15 |
Exercises C, D: analyzing ligations making competent cells
|
Paper 1: Cohen subcloning paper |
| Sept. 20 |
Exercise E: transformations |
|
| Sept. 21 |
Exercise F : starting DNA minipreps |
|
| Sept. 22 |
Exercise G, H : Minipreps, restriction digests and gels |
|
| Sept. 27 |
PROJECT 2 & 3 LAB MEETING |
Lab meeting topic-Transformation of different species and vectors |
| Sept. 29 |
Project 4: DNA sequencing: Exercise A PCR |
Prelab Project 4 due, READ Primrose pp 120-132 |
| Oct. 4 |
Exercise B : DNA purification |
|
| Oct. 6 |
Introduction to the BLC and Bioinformatics |
Lab report projects 2 and 3 due, 5 page max
Tour of sequencing center, Section 1 |
| Oct. 11 |
DNA sequence analysis |
|
| Oct. 13 |
DNA sequence analysis |
|
| Oct. 18 |
PROJECT 4 LAB MEETING/review session
|
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| Oct. 20 |
MIDTERM EXAM: Projects 2,3,4 |
Projects 2, 3 and 4, 2 hrs MAX |
| Oct. 25 |
Project 5: Defining a gene: A: Purifying DNA, PCR |
Prelab project 5 due,READ Primrose pp 19-25, 95-102 |
| Oct. 27 |
B:Purifying RNA |
|
| Nov. 1 |
C: making cDNA |
RACE protocol |
| Nov. 3 |
D:Tailing cDNA and PCR |
Paper 2: Microarrays in Strawberries |
| Nov. 8 |
E: Gel purification and ligation |
Ligation and transformation protocol |
| Nov. 10 |
F: Transformation |
Tour of Microarray facility |
| Nov. 12 |
G: start minipreps |
|
| Nov. 15 |
H: Minipreps and digests/gel |
review old miniprep protocol and digests |
| Nov. 17 |
Project 6: A Introduction to protein expression (pet-23 map) |
Prelab project 6 due, READ Primrose pp 73-84 |
| Nov. 22 |
Project 5: sequence analysis worksheet |
|
| Nov. 24 |
Thanksgiving Holiday-no class |
|
| Nov. 29 |
Protein purification and gels |
|
| Dec. 1 |
Finish Protein expression
Tour of Mass-Spec facility? |
Lab report projects 4 and 5 due (5 page max) |
| Dec. 6 |
Review of course, lab meeting project 5 |
|
| Dec. 8 |
Final |
Lab report Project 6 due (1 page max) |
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